The Municipal Library of Tyrnavos is home to an important collection of Greek manuscripts, which was gradually composed from the 17th century onwards based on the book collection of the renowned School of Tyrnavos. It comprises forty seven codices containing mostly works related to education (theology, grammar, mathematics, astronomy, medicine, etc) and texts for use in churches and monasteries, such as the Old Testament, the Gospels, Nomocanons, Patristic Homilies, and ascetic narratives. Chronologically, they date from the 10th to the 19th century, while certain are quite important in terms of content, their own history, writing or illumination. The present catalogue covers a gap in the research of the field, as until now scholars have only made short mentions of the manuscripts of the collection, six of which remaining totally unknown. The description of each volume is thorough, and follows the latest international standards in manuscript cataloguing, with a detailed description of content, an analysis of technical specifications, both palaeographical and codicological, a transcription of all existing notes, and related bibliography.